Effect of Temperature and storage time on Hepatobiliary enzyme activities in Goat serum

The present study was designed and conducted to choose an ideal storage condition for goat sera samples meant for the assay of hepatobiliary enzymes such as, alanine aminotransferases(ALT), aspartate aminotransferases (AST), alkaline phosphatase (ALP) and gamma glutamyltransferases (GGT) by storing at room temperature, 4 oC and -20 oC up to 14 days. Gamma glutamyltransferase was found to be the most stable enzyme in all the three storage conditions through out the study period. Alanine aminotransferase was stable only up to 8 days at 4 oC whereas marked stability was noticed at -20 oC and room temperature as long as 14 days. Aspartate aminotransferase was more stable at -20 oC up to14 days and 11 days at 4 oC whereas at room temperature only 2 days. Alkaline phosphatase showed great variation upon storage as compared to other hepatobiliary enzymes and it is suggested that its estimation should be performed in fresh serum samples to get a more accurate result. From these results it is therefore advisable to consider stability of each serum hepatobiliary enzymes for different animals separately before preserving sera samples to get more valid and reliable result.